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RNAi-based functional genomics & phenomics project

Published on 20 August 2020
Project leader

Éric Sulpice
Laboratoire Biologie à Grande Échelle
Biomics team
CEA-Grenoble
17, avenue des Martyrs, 38 054 Grenoble
Phone: 33 (0)4 38 78 25 90
e-mail: eric.sulpice@cea.fr

Our laboratory develops innovative micro and nanotechnologies to perform and optimize cell-based small interfering RNA (siRNA), miRNA mimics and inhibitors (LNA) functional screens, in physiologically relevant formats (3D cultures, single cell, primary cells, and FACS-based screens).

We cover the entire pipeline to perform robust RNAi screens:




Cell culture models

2D human cell lines or primary cells
 
On large micropatterns (from 100 to 400µm)


On single cell micropatterns
 
3D human cell lines


RNAi-based HTS-HCS screening facilities







Collaborations

- Imperial College London
- Gustave Roussy Institute
- Genome Institute of Singapore
- Centre Léon Bérard Lyon
- Cinvestav


Reference

Agaesse G, Barbollat-Boutrand L, Sulpice E, Bhajun R, Kharbili ME, Berthier-Vergnes O, Degoul F, de la Fouchardiere A, Berger E, Voeltzel T, Lamartine J, Gidrol X and Masse I
A large-scale RNAi screen identifies LCMR1 as a critical regulator of Tspan8-mediated melanoma invasion.
Oncogene, 2017, 36(4): 446-457

For an exhaustive list see Publications