Thesis presented May 05, 2008
Abstract:
Using a photochemical grafting method for bio-molecules in a capillary, developed by the Biopuces Laboratory at the CEA, we have developed a protein biochip in an innovating format for multiparametric serodiagnosis assays. This grafting method allows, on the internal wall of the capillary, the covalent and localized binding of protein antigens which will react with patient serums during the immunoassays.
The first part of this work presents the methods implemented for the grafting of the antigens on the inner capillary surface, with the aim of an industrializable method for a multiparametric diagnosis tool for bacterial infections. We have shown that the sequential method is the only method which fits to Abag specified characteristics.
The second part presents the assessment of the capillary immunoassay performances. In a first part, we have estimated and compared the individual performance of each antigen bound in a capillary versus the performance of the same antigen adsorbed for an ELISA test. Preliminary results show that the capillary arrays are at least as effective as ELISA tests. Then, we have underlined the efficiency of the antigen capillaries for reliable multiparametric serodiagnosis tests.
Keywords:
Biochip, capillary, immnuoassay, multiparametric test, infectious diagnosis, photochemistry, antigen, antibody, microfluidics