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A tissuloid model for studying pancreatic morphogenesis and carcinogenesis

Published on 14 October 2022
Objective of the internship
The project aims to study a unique surrogate tissue model (tissuloid) developed in the laboratory. The tissuloids are formed by self-assembly of pancreatic cells under 3D culture conditions. This process resembles pancreatic morphogenesis with the formation of macroscopic epithelial intermediates typical of embryonic organs. We have shown that tissuloids retain such key features of pancreatic tissue as polarized epithelium architecture and expression of specific markers, and therefore, represent a promising model for translational research and drug testing. 

Specifically to the M2 internship, the goal will be to optimize 3D cell culture conditions and to perform morphological and functional characterization of pancreatic tissuloids.

Activities assigned
The intern will (i) perform 3D cell culture; (ii) analyze tissuloid growth and morphology using a variety of microscopy techniques; (iii) analyze the expression of specific markers using immuno-fluorescence microscopy, western blotting, and RNA sequencing (iv) characterize tissuloids formed by different types of cells including immortalized cell lines, primary cells, and cells issued from organoid cultures. The intern will also be involved in initial characterization of tissuloid metabolism by using organ-on-a-chip (OSP) tools.

Skills to be acquired or developed
In addition to general bench experience, the intern will acquire skills in 3D cell culture, fluorescence microscopy, and western blotting.

Other information 
Laboratory: Biomics team, IRIG, CEA Grenoble (UGA-Inserm-CEA) 17 avenue des Martyrs, 38 054 Grenoble.
Contact: Maxim Balakirev